Mesenchymal stem cell derived exosomes : the potential for translational nanomedicine / edited by Yaoliang Tang, Buddhadeb Dawn.

Includes bibliographical references and index.

Cover; Title Page; Copyright Page; Contents; List of Contributors; Preface; Chapter 1 -- Insights into the Mechanism of Exosome Formation and Secretion; 1 -- Introduction; 2 -- Signalings in the regulation of endocytosis; 3 -- Signalings in the regulation of early endosome; 4 -- Signalings in the regulation of exosome cargo loading to late endosomes; 5 -- Signalings in the regulation of the fate of multivesicular bodies (MVBs); 6 -- Signalings in the regulation of exosome release; 7 -- Other signalings in the regulation of exosome biogenesis and release

8 -- Stress and pathologic conditions can stimulate exosome release9 -- Concluding Remarks; Abbreviations; References; Chapter 2 -- An Overview of the Proteomic and miRNA Cargo in MSC-Derived Exosomes; 1 -- Background; 1.1 -- Mesenchymal Stem Cells; 1.2 -- Mechanism of Action of MSCs: Paracrine Secretion; 1.3 -- Secreted MSC Mediators; 1.4 -- Active Agent in MSC Secretion: Exosome; 2 -- Cargo of MSC exosomes; 2.1 -- RNA Cargo of MSC Exosomes; 2.2 -- Proteome of MSC Exosomes; 2.2.1 -- Computational Analysis of the MSC Exosome Proteome; 2.2.2 -- Biochemical Activity of the MSC Proteome; 2.2.3 -- Enzymes

2.2.4 -- Inhibitors2.2.5 -- Immune Modulators; 3 -- Conclusions; Abbreviations; References; Chapter 3 -- Exosome Function in miRNA-Mediated Paracrine Effects; 1 -- Introduction; 2 -- Physical and molecular compositions; 2.1 -- Isolation of Exosomes; 2.2 -- Biogenesis of Exosomes; 2.3 -- Characterization of Exosomes; 2.4 -- Functions of Exosomes; 3 -- MicroRNAs (miRNAs) in exosomes; 3.1 -- Paracrine Effects of miRNAs; 4 -- Exosomes and diseases; 4.1 -- Cardiovascular Diseases; 4.2 -- Neurodegenerative Diseases; 4.3 -- Renal Diseases; 4.4 -- Exosomes in Cancer; 4.5 -- Pulmonary Diseases

4.6 -- Stem Cells and Exosomes5 -- Future Perspective; 6 -- Conclusions; Abbreviations; References; Chapter 4 -- Current Methods to Purify and Characterize Exosomes; 1 -- Introduction to exosomes; 1.1 -- Defining an Exosome; 1.2 -- Organization of this Chapter; 2 -- Isolation of exosomes; 2.1 -- Preparing Exosome Collection Media; 2.1.1 -- Materials; 2.1.2 -- Protocol; 2.2 -- Basic Ultracentrifugation Protocol; 2.2.1 -- Materials; 2.2.2 -- Protocol; 2.2.2.1 -- Removal of Cells, Dead Cells, and Cell Debris; 2.2.2.2 -- Purify Exosome Fraction; 2.2.2.3 -- Wash Exosomes; 2.3 -- Alternative Procedures

2.3.1 -- Materials2.3.2 -- Protocol; 2.4 -- Special Case Ultracentrifugation Protocol; 2.4.1 -- Materials; 2.4.2 -- Protocol; 2.5 -- High Purity Ultracentrifugation Protocol; 2.5.1 -- Materials; 2.5.2 -- Protocol; 2.6 -- Immunoisolation Protocol; 2.6.1 -- Materials; 2.6.2 -- Protocol; 2.6.2.1 -- Immunoprecipitate Exosomes; 3 -- Characterization of exosomes; 3.1 -- Imaging of Exosomes by Electron Microscopy; 3.1.1 -- Materials; 3.1.2 -- Protocol; 3.1.2.1 -- Fixing Exosomes on EM Grids; 3.1.2.2 -- Contrast and Embed Exosome Samples; 3.2 -- Identifying Exosome Proteins by Immunogold Labeling; 3.2.1 -- Materials

3.2.2 -- Protocol

Online resource; title from PDF title page (EBSCO, viewed September 10, 2015)

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