Atlas of oral microbiology: from healthy microflora to disease /
Xuedong Zhou, Yuqing Li, editors.
- 2nd ed.
- Singapore : Springer, 2021.
- 1 online resource (368 p.)
2.3.4 Confocal Laser Scanning Microscopy. Includes index.
Intro -- Preface -- Contents -- Editors and Contributors -- Chapter 1: Basic Biology of Oral Microbes -- 1.1 Cytological Basis of Microorganisms -- 1.2 Microbial Morphology -- 1.2.1 Microbial Size -- 1.2.2 Microbial Morphology -- 1.3 Microbial Cell Structure -- 1.3.1 Basic Bacterial Structures -- 1.3.1.1 Cell Wall -- 1.3.1.2 Cell Wall-Deficient Bacteria (Bacterial L Form) -- 1.3.1.3 Cell Membrane -- 1.3.1.4 Cytoplasm -- 1.3.1.5 Nuclear Material -- 1.3.2 Special Bacterial Structures -- 1.3.2.1 Capsule -- 1.3.2.2 Flagellum -- 1.3.2.3 Pilus -- 1.3.2.4 Spore -- 1.3.3 Basic Virus Structure 1.4 Microbial Physiology -- 1.4.1 Binary Fission Reproduction -- 1.4.2 Bacterial Growth -- 1.5 Microbial Genetics -- 1.5.1 Heredity -- 1.5.2 Variation -- 1.5.3 Genetic Material of Bacteria -- 1.5.4 Mechanism of Microbial Variation -- 1.5.5 Gene Transfer and Recombination -- 1.5.5.1 Transformation -- 1.5.5.2 Conjugation -- 1.5.5.3 Transduction -- References -- Chapter 2: Techniques for Oral Microbiology -- 2.1 Smear and Stain Techniques -- 2.1.1 Kopeloffś Modification of Gram Stain -- 2.1.1.1 Mechanism -- 2.1.1.2 Preparation of Gram Stain Reagents (Commercially Available) 2.1.1.3 Staining Procedure -- 2.1.1.4 Reporting Results -- 2.1.1.5 Precautions -- 2.1.2 Staining Bacterial Spores -- 2.1.2.1 Gram Stain -- 2.1.2.2 Fuchsin-Methylene Blue Stain -- 2.1.3 Staining the Bacterial Capsule -- 2.1.3.1 Murs Stain -- 2.1.3.2 Wrightś Stain -- 2.1.4 Flagellar Stain -- 2.1.4.1 Preparation of Staining Solutions -- 2.1.4.2 Smear Preparation -- 2.1.4.3 Staining Protocol -- 2.1.5 Staining Procedure for Special Fungal Structures -- 2.1.5.1 Crystal Violet Stain -- 2.1.5.2 Lactophenol Cotton Blue Stain -- 2.1.6 Giemsa Stain for Mycoplasma 2.1.7 Negative Congo Red Staining of Plaque Bacteria -- 2.1.8 Protozoan Smears -- 2.1.8.1 Wet Smear for Fresh Samples -- 2.1.8.2 Giemsa Staining -- 2.2 Isolation, Incubation, and Identification Techniques -- 2.2.1 Collection and Transportation of Samples -- 2.2.1.1 Sample Collection -- 2.2.1.2 Sample Transportation -- 2.2.2 Suspension and Dilution of Samples -- 2.2.2.1 Sample Suspension in Solution -- 2.2.2.2 Sample Dilution -- 2.2.3 Inoculation and Incubation of Samples -- 2.2.3.1 Choice of Medium -- 2.2.3.2 Sample Inoculation -- 2.2.3.3 Sample Incubation 2.2.4 Growth Characteristics and Identification -- 2.2.4.1 Growth Characteristics -- 2.2.4.2 Biochemical Tests -- 2.2.5 Instruments for Microbiological Identification -- 2.2.5.1 Spiral Plater -- 2.2.5.2 Microbiology Analyzer -- 2.2.5.3 Microbial Identification System -- 2.3 Microscopy Techniques -- 2.3.1 Stereomicroscopy -- 2.3.2 Scanning Electron Microscopy -- 2.3.2.1 Mechanism -- 2.3.2.2 Operating Procedure -- 2.3.2.3 Sample Observation -- 2.3.3 Transmission Electron Microscopy -- 2.3.3.1 Preparation of Bacterial Samples -- 2.3.3.2 Section -- 2.3.3.3 Uranium Staining and Observation
This book is the second edition of Atlas of Oral Microbiology: From Healthy Microflora to Disease (ISBN 978-0-12-802234-4), with two new features: we add about 60 pictures of 14 newly isolated microbes from human dental plaque, at the same time, we re-organize the content of this book and provide more research progress about the oral microbiome bank of China, the invasion of oral microbiota into the gut, and the relationships between Oral Microflora and Human Diseases. This book is keeping up with the advanced edge of the international research field of oral microbiology. It innovatively gives us a complete description of the oral microbial systems according to different oral ecosystems. It collects a large number of oral microbial pictures, including cultural pictures, colonies photos, and electron microscopy photos. It is by far the most abundant oral microbiology atlas consists of the largest number of pictures. In the meantime, it also described in detail a variety of experimental techniques, including microbiological isolation, culture, and identification. It is an atlas with strong practical function. The editors and writers of this book have long been engaged in teaching and research work in oral microbiology and oral microecology. This book deserves a broad audience, and it will meet the needs of researchers, clinicians, teachers, and students major in biology, dental medicine, basic medicine, or clinical medicine. It can also be used to facilitate teaching and international academic exchanges.
9789811578991 RM 2,003.93 9811578990
10.1007/978-981-15-7899-1 doi
Mouth--Microbiology--Atlases. Mouth--microbiology. Dentistry. Medical microbiology. Microbiology. Mouth--Microbiology.