PCR detection of microbial pathogens / Polymerase chain reaction detection of microbial pathogens. edited by Mark Wilks. - Second Edition. - xi, 317 pages : illustrations (some colour) ; 26 cm. - Methods in Molecular Biology, 943. 1064-3745 ; Springer protocols. . - Methods in molecular biology (Clifton, N.J.) ; 943. Springer protocols. .

Previous edition : 2003. Buku Bacaan Asas Fakulti.

Includes bibliographical references and index.

Real-time quantitative PCR, pathogen detection and MIQE / Overcoming inhibition in real-time diagnostic PCR / Quality in the molecular microbiology laboratory / Pre-analytical sample treatment and DNA extraction protocols for the detection of bacterial pathogens from whole blood / Detection of bacterial contamination in platelet concentrates using flow cytometry and real-time PCR methods / Multiplex real-time PCR assay for the detection of meticillin-resistant Staphylococcus aureus and Panton-Valentine leukocidin from clinical samples / PCR Detection of Haemophilus influenzae from respiratory specimens / Rapid detection of atypical respiratory bacterial pathogens by real-time PCR / Utilization of multiple real-time PCR assays for the diagnosis of Bordetella spp. in clinical specimens / Detection of Mycoplasma pneumoniae by real-Time PCR / Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia / Rapid identification of mycobacteria and rapid detection of drug resistance in Mycobacterium tuberculosis in cultured isolates and in respiratory specimens / Direct detection of Mycobacterium ulcerans in clinical specimens and environmental samples / Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay / Simultaneous direct identification of genital microorganisms in voided urine using multiplex PCR-based reverse line blot assays / Diagnosis of Clostridium difficile infection using real-time PCR / Detection of pathogenic Leptospira spp. through real-time PCR (qPCR) targeting the LipL32 gene / Sensitive and rapid detection of Campylobacter jejuni and Campylobacter coli using loop-mediated isothermal amplification / PCR detection of Helicobacter pylori in clinical samples / Rapid detection of the Escherichia coli genospecies in water by conventional and real-time PCR / Multiplex real-time PCR (MRT-PCR) for diarrheagenic / Gemma Johnson, Tania Nolan, and Stephen A. Bustin -- Johannes Hedman and Peter Rstr语 -- Paul S. Wallace and William G. MacKay -- Wendy L.J. Hansen, Cathrien A. Bruggeman, and Petra F.G. Wolffs -- Tanja Vollmer, Knut Kleesiek, and Jens Dreier -- Lynne Renwick, Anne Holmes, and Kate Templeton -- Guma M.K. Abdeldaim and Bj诲n Herrmann -- Clare L. Ling and Timothy D. McHugh -- Kathleen M. Tatti and Maria Lucia Tondella -- Jonas M. Winchell and Stephanie L. Mitchell -- Judith Fillaux and Antoine Berry -- Wing-Cheong Yam and Kit-Hang Gilman Siu -- Caroline J. Lavender and Janet A.M. Fyfe -- Anneke M.C. Bergmans and John W.A. Rossen -- Michelle L. McKechnie, Fanrong Kong, and Gwendolyn L. Gilbert -- Renate Johanna van den Berg, Dennis Bakker, and Ed J. Kuijper -- Robyn Anne Stoddard -- Wataru Yamazaki -- Emiko Rimbara, Masanori Sasatsu, and David Y. Graham -- Andr F. Maheux, Luc Bissonnette, and Michel G. Bergeron -- Francesca Barletta, Theresa J. Ochoa, and Thomas G. Cleary.

9781603273527 RM587.95 1603273522


Diagnostic microbiology.
Polymerase chain reaction--Diagnostic use.
Molecular diagnosis.
Diagnostic Techniques and Procedures--Laboratory Manuals.