DNA microarrays for biomedical research : methods and protocols /
edited by Martin Dufva
- New York : Humana Press, c2009.
- ix, 304 p. : ill. ; 27 cm.
- Methods in molecular biology, 529 1064-3745 ; .
- Methods in molecular biology (Clifton, N.J.) ; 529. .
Includes bibliographical references and index.
Introduction to microarray technology / Martin Dufva -- Probe design for expression arrays using OligoWiz / Rasmus Wernersson -- Comparative genomic hybridization : microarray design and data interpretation / Richard Redon and Nigel P. Carter -- Design of tag SNP whole genome genotyping arrays / Daniel A. Peiffer and Kevin L. Gunderson -- Fabrication of DNA microarray / Martin Dufva -- Immobilization chemistries / Sascha Todt and Dietmar H. Blohm -- Fabrication using contact spotter / Annelie Walden and Peter Nilsson -- RNA preparation and characterization for gene expression studies / Michael Stangegaard -- Gene expression analysis using Agilent DNA microarrays / Michael Stangegaard -- Target preparation for genotyping specific genes or gene segments / Jesper Petersen, Lena Poulsen, and Martin Dufva -- Genotyping of mutations in the beta-globin gene using allele specific hybridization / Lena Poulsen, Jesper Petersen, and Martin Dufva -- Microarray temperature optimization using hybridization kinetics / Steve Blair ... [et al.] -- Whole-genome genotyping on bead arrays / Kevin L. Gunderson -- Genotyping single nucleotide polymorphisms by multiplex minisequencing using tag-arrays -- Lili Milani and Ann-Christine Syvanen -- Resequencing arrays for diagnostics of respiratory pathogens / Baochuan Lin and Anthony P. Malanoski -- Comparative genomic hybridization : DNA preparation for microarray fabrication / Richard Redon, Diane Rigler, and Nigel P. Carter -- Comparative genomic hybridization : DNA labeling, hybridization, and detection / Richard Redon, Tomas Fitzgerald, and Nigel P. Carter -- Chromatin immunoprecipitation using microarrays / Mickael Durand-Dubief and Karl Ekwall